TITLE:
Genealogy of Cucumber mosaic virus Isolated from Ornamental Species
AUTHORS:
Ligia Maria Lembo Duarte, Eliana Borges Rivas, Ricardo Harakava, Maria Cristina Duchene Veauvy, Maria Amelia Vaz Alexandre
KEYWORDS:
Phylogeny; Maximum Likelihood; Molecular Characterization; CMV; Ornamentals
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.5,
May
29,
2013
ABSTRACT:
Cucumber mosaic virus (CMV) has the broadest host range, infecting more than 1300 species in
more than 500 genera from over 100 botanical families. In ornamental plants, CMV can cause mosaic and
distortion of leaves,
stunting, color break, and malformation of flowers.
CMV coat protein (CP) sequences obtained from seven ornamental plants and other
homologous sequences available in GenBank were compared, and phylogenetic
relationships were established. Total RNA from virus-infected ornamental
species were extracted, submitted to RT-PCR with specific primers, and
amplicons obtained were sequenced. A nucleotide substitution model and
phylogenetic analyses were carried out using the PAUP program. The seven sequences of
CMV CP obtained showed similar identity percentages and close relationships
with subgroup I isolates from other countries and hosts. CMV isolates from different
regions of S?o Paulo state,
Brazil (Salvia splendens, Catharanthus
roseus, Nematanthus nervosus (=Hypocyrta nervosa), Impatiens walleriana, Eucharis grandiflora and Commelina sp.) formed a monophyletic group,
indicating a possible common origin. It was found that when lily sequences of CMV
only from different geographic regions were compared, Brazilian isolates shared
the same common ancestor with those from Poland and Taiwan.
Furthermore, this monophyletic group presented a quite basal position.