TITLE:
Detection of 232bp Virulent Gene of Pathogenic Aeromonas hydrophila through PCR Based Technique: (A Rapid Molecular Diagnostic Approach)
AUTHORS:
Agniswar Sarkar, Mousumi Saha, Pranab Roy
KEYWORDS:
Aeromonas Spp.; Hemolysin Gene; AHCYTONE; Molecular Diagnosis; PCR
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.1,
March
28,
2013
ABSTRACT:
The pathogenicity of aeromonads produces due to exotoxins such as
cytolytic enterotoxin, hemolysin or aerolysin, lipases and
proteases. Rapid detection of A.
hydrophila cytolytic enterotoxin (AHCYTONE) gene and their characterization
has proven importance so that proper and rapid preventive and control measures
could be taken up to reduce mortality and loss in fish culture. The main
objective of the present study is to genetic identification of AHCYTONE positive Aeromonas hydrophila. Strains were isolated from fishes from different
fish market in West Bengal and water samples from
different river and ponds. Initially strains were identified by their
phenotypic and biochemical characterization. Due to
contradiction of those results, molecular characterization was done by
polymerase chain reaction, which is proved a suitable and rapid diagnostic tool
for identification and characterization of A.
hydrophila. We have also evaluated the potential risk to human health that
this finding can represent by determining the presence of the cytolytic enterotoxin
gene in such isolates.