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A. N. Sharpe, “Food Sample Preparation and Enrichment for Rapid Detection,” In: S. T. Clarke, K. C. Thompson, C. W. Keevil and M. S. Smith, Eds., Rapid Detection Assays: For Food and Water, Royal Society of Chemistry, Cornwall, 2001, pp. 129-137. doi:10.1039/9781847551818-00129

has been cited by the following article:

  • TITLE: A Resistance Based Biosensor That Utilizes Conductive Microfibers for Microbial Pathogen Detection

    AUTHORS: Shannon K. McGraw, Evangelyn Alocilja, Kris Senecal, Andre Senecal

    KEYWORDS: Electrotextile; Biosensor; Polypyrrole; Immunosensor; Resistance Based Detection

    JOURNAL NAME: Open Journal of Applied Biosensor, Vol.1 No.3, November 21, 2012

    ABSTRACT: Escherichia coli O157:H7 (E. coli O157:H7) is one of the top pathogens of interest for the development of rapid diagnostic systems for food and water samples. The objective of this research is to develop a rapid, novel electrochemical biosensor based on the use of polypropylene microfiber membranes coated with a conductive polypyrrole and antibody functionalized for the biological capture and detection of E. coli O157:H7 inthe field. Using glutaraldehyde, pathogen specific antibodies are covalently attached to conductive microfiber membranes which are then blocked using a 5% bovine serum albumin solution. The functionalized membranes are then exposed to E. coli O157:H7 cells washed in Butterfield’s phosphate buffer and added to a phosphate-buffer electrolyte solution. When a voltage is applied to the system, the presence of the captured pathogen on the fiber surface results in an increase in resistance at the electrotextile electrode surface, indicating a positive result. In this study, the initial resistance of the membrane in the electrochemical system was established and found to range between 5.8 and 13 . The resistance of the system not associated with the electrotextile fibers was calculated to contribute to only 2.8% of the total system resistance, and found not to be significant. A proof of concept experiment was conducted and determined that the electrotextile electrode was able to differentiate between small changes in a solution’s conductivity associated with the presence of E. coli O157:H7 cells over a concentration range of log 0 - 9 CFU/mL.