Jia and Bonierbale, “Validation of Conserved Orthologous Markers, A Proposal for Extension of Commissioned Research in Subprogram 2 ‘Comparative Genomics’ Cluster 2: Marker Development Generation Challenge Program,” 2005.

has been cited by the following article:

• TITLE: Transferability of Sorghum Genic Microsatellite Markers to Peanut

  AUTHORS: Siddanna B. Savadi, Bashasab Fakrudin, H. L. Nadaf, M. V. C. Gowda

  KEYWORDS: Arachis Hypogea; Transferability; EST-SSR; Polymorphism

  JOURNAL NAME: American Journal of Plant Sciences, Vol.3 No.9, September 26, 2012

  ABSTRACT: Currently development of new marker types has shifted from anonymous DNA fragments to gene-based markers. Simple Sequence Repeats (SSRs) are useful DNA markers in plant genetic research including in peanut. However, de novo development of SSRs is expensive and time consuming. Gene-based DNA markers are transferable among related species owing to the conserved nature of genes. In this study transferability of sorghum EST-SSR (SbEST-SSR) markers to peanut was prospected. A set of 411 SbEST-SSR primer pairs were used to amplify peanut genomic DNA extracted from cultivated peanut where 39% of them successfully amplified. A comparison of amplification patterns between sorghum and peanut showed similar banding pattern with majority of transferable SbEST-SSRs. Among these transferable SSR markers, 14% have detected polymorphism among 4 resistant and 4 susceptible peanut lines for rust and late leaf spot diseases. These transferable markers will benefit peanut genome research by not only providing additional DNA markers for population genetic analyses, but also allowing comparative mapping to be possible between peanut and sorghum—a possible monocot-dicot comparison.