TITLE:
Genetic Diversity in Jatropha platyphylla Accessions Based on Morphological Traits and Inter-Simple Sequence Repeats Molecular Markers
AUTHORS:
Edith Salazar-Villa, Lilia Alcaraz-Meléndez, Josefina León-Félix, José Basilio Heredia, Federico Soto-Landeros, Veronica Pérez-Rubio, Eduardo Sánchez-Valdez, Miguel Angulo Angulo-Escalante
KEYWORDS:
Fatty Acids, Oil, Molecular Markers, Polymorphism, Plant Breeding
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.11,
November
23,
2021
ABSTRACT: Seven accessions of Jatropha platyphylla were evaluated
for their phenotypic traits and genetic diversity using inter-simple sequence
repeats (ISSRs). Cluster analyses with nine traits were performed: number of
branches per plant; fruit per bunch; bunch per branch; bunch per plant; total
seed production; total fruit production, protein content, oil content, and
fatty acid profile. Genotypes from Rosario, Sinaloa, Mexico (PR11) yielded the
highest values in all traits. The
correlation analysis of the quantitative traits showed high correlations
between seed and total fruit production (r = 0.99). Unsaturated linoleic acid
was the most abundant fatty acid (57.64% - 52.39%). Within a genetic improvement
program, two of the most important variables to be considered are oil content
and phenotypic characteristics of the plant. J. platyphylla has shown viable selection traits that
provide a possibility of producing interspecies hybrids and giving them added
value. ISSRs primers generated variable banding patterns that were found to be
polymorphic; the polymorphic information content (PIC) of these loci ranged
from 0.21 to 0.45 with an average of 0.34. The unweighted pair group method
(UPGMA) cluster analysis of the data showed the formation of three groups,
where the most divergent accession pair was the genotype from Quelite (QP11)
and Rosario (PR11).