TITLE:
Characterization of Endophytes Isolated from Eclipta prostrate and Roles in Regulating the Gut Microbiota of C57BL/6J Mice
AUTHORS:
Xin Zhao, Huishu Shi, Yajing Wang
KEYWORDS:
Endophyte, Eclipta prostrata, Gut Microbiota, C57BL/6J Mice, Lactococcus
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.11 No.5,
May
14,
2020
ABSTRACT:
Eclipta prostrata has been extensively studied
for its promoting effect on human health.
Previous study proved that E. prostrata possessed anti-osteoporosis function in SAMP6 mice via gut microbiota (GM). Endophytes are widespread in
plants, especially in Chinese herbal medicines. However, little is known regarding
the endophytes of E. prostrata. In this study, we focus on screening and identifying the endophytes
from plant E. prostrata and exploring their roles in modulating GM. According to biochemical,
physiological tests and 16S rDNA sequence analysis, ten
endophytes were characterized in different locations of plant E. prostrata belonging to Lactococcus, Bacillus, Enterococcus, Exiguobacterium and Pantoea. The antimicrobial
activity of endophytes EP01-10 was investigated via the oxford cup method.
Furthermore, the acid and bile salt resistant ability of EP01-10 was detected
to explore their survival ability in gastrointestinal tract. Results indicated
that strains of Lactococcus, Bacillus and Exiguobacterium (EP01, 03 and 05) showed strong antimicrobial activity against Escherichia coli and Staphylococcus aureus and strains of Lactococcus, Bacillus and Enterococcus (EP01, 02, 04, 08
and 10) have the ability to survive in the gastrointestinal tract. 16S rRNA
sequencing of GM in C57BL/6J mice was performed for diversity and structure
analysis responded to the administration of E. prostrata extract. E. prostrata extract acted on improving the microflora diversity, particularly in increasing
the abundance of Lactococcus significantly. Thus, as an endophyte, Lactococcus plays an important role in E. prostrata modulating intestinal tract. Our study suggested that endophytes of Chinese
herbal medicines might be a novel target for the treatment of diseases by
regulating the GM.