TITLE:
Diagnosis of the Bio-Compound as an Anti-Fungal from Populus euphratica L. Plant Using Chromatographic Technology
AUTHORS:
Rana Ibrahim Khaleel
KEYWORDS:
Populus euphratica, Secondary Metabolites, LC-MS, Antifungi, Alpha Glycoside
JOURNAL NAME:
Open Journal of Ecology,
Vol.9 No.5,
May
27,
2019
ABSTRACT:
Crude of
Plant extracts from materials is necessary screening to determine new
biological activities, toxicity and active compounds in bioassay tests. Crude of Populus
euphratica L. left is tested to phytochemical screening, constituents, fractionation and MIC
technique as anti-fungal. The fractions are subjected to bio-compound analysis using Liquid chromatography-mass spectrometry LC-MS. Phytochemical screening of crude extract shows
presence of Glycosides compared with other compounds. While the fraction of
ethanolic extract recorded inhibition impact reached (390, mg/L/MIC) agnist
type of fungi. Bioassay toxicity using shrimp lethally test (LC50)
was carried out with the result showing 4.1 mg/L is low toxic and can be used safely. Alpha
Glycoside (4, 6 Benzylidene-1O-Methyl-2O-(2346 Tetra-O-Acetyl-Betad- Glucosyl), is a new compound and antifungal
action.