TITLE:
A Simplified Approach for Olive (Olea europaea L.) Genotyping and Cultivars Traceability
AUTHORS:
L. Braglia, A. Manca, S. Gianì, P. Hatzopoulos, Diego Breviario
KEYWORDS:
Olive, Genotyping, Tubulin Based Polymorphism, SSRs, Molecular Probes
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.13,
December
25,
2017
ABSTRACT: The Tubulin Based Polymorphism (TBP) method was used
to genotype olive cultivars of different origin and to produce short-size cultivar-specific
molecular probes. Both the first and the second intron of the members of the
olive β-tubulin gene family were
exploited as sources of DNA polymorphism. Compared with the data obtained with
the use of a set of 11 SSR markers selected from an Olea europea L. database, TBP is shown to provide similar, if not
better, information about the polymorphic content of the olive genomes,
releasing, at the same time, a simple and discriminatory DNA barcode specific
for any of the analyzed cultivars. Such a barcode is the source for the
preparation of variety specific molecular probes.