TITLE:
Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector
AUTHORS:
Florence Hubert, Céline Loiseau, Françoise Ergan, Gaëlle Pencréac’h, Laurent Poisson
KEYWORDS:
Free Fatty Acids, HPLC, Evaporative Light-Scattering Detector, Core-Shell Technology, Method Validation
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.8 No.12,
December
4,
2017
ABSTRACT:
A high-performance liquid chromatography analysis method with an evaporative
light-scattering detector has been developed for the separation and
quantitative analysis of free fatty acids in biological matrices. Core-shell reversed-phase high-performance liquid chromatography separation of 10 free
fatty acids is achieved within 10.5 min using a methanol/water (0.05% trifluoroacetic
acid) eluent gradient. After optimization, the drift tube and nebulization
temperature of the evaporative light-scattering detector was set at 35°C, nitrogen
flow-rate at 1.1 standard liter per minute and column temperature at
25°C. All calibration curves showed good regression (r2 > 0.9975). A validation
procedure following the International Conference on Harmonisation guidelines
was implemented to certify the method. Relative standard deviations did not
exceed 1.5% and 4.25% for repeatability and reproducibility respectively.