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has been cited by the following article:

• TITLE: Rapid Screening of Recombinant Plasmids by Direct Colony Quantitative Real-Time PCR

  AUTHORS: Lei Hou, Xiuying Zhang, Yang Li, Shuai Chen, Hongyi Qu, Jiazhi Yu, Lianhai Zhang, Ziyi Fan

  KEYWORDS: qPCR, Colony PCR, Plasmid Screening

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.7 No.10, October 31, 2016

  ABSTRACT: Quantitative real-time PCR (qPCR) was applied to rapid screening of positive plasmid clones. Insert-specific primer pairs were used in qPCR colony screening, and false positive colonies could easily be distinguished from true positive ones by comparing their Ct values. In addition, qPCR is particularly suitable when amplicon is small (