TITLE:
Study of the Reactions of Microorganisms in Response to the Magnetite Nanoparticles
AUTHORS:
Andrey Belousov, Yulia Voyda
KEYWORDS:
Magnetite Nanoparticles, Microorganisms, Free Radicals Peroxidation Lipids, Polarization Structure, Bacteriostatic Effect
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.4,
April
22,
2015
ABSTRACT:
The influence of basic physical factors caused by magnetite nanoparticles
(constant magnetic field and sorption) on microorganisms by examining the reactions
of the intensity of free radical lipid peroxidation (FRLP) and bacteriostatic action
was studied. It was well established that the magnetite nanoparticles caused unequal
reaction in intensity of FRLP on different groups of microorganisms. It was determined
that the most significant factor that influenced on the ultimate indicator of the
intensity of luminescence on C. albicans, E. coli and P. aeruginosa was constant magnetic field which induced by nanoparticles.
On the contrary, sorption was the most significant factor on S. aureus. It was found that the rate of
consumption of free radicals lipid reduced reliably on all microorganisms after
their processing by magnetite nanoparticles. The results of microbiological
studies of E. coli, K. pneumoniae and S. aureus showed that bacteriostatic effect was detected after
exposure by magnetite nanoparticles. Visually, it was detected by decreasing the
number of colonies on the nutritious medium in comparison with the control. It was
revealed an interesting fact that saline NaCl, which had previously been processed
by magnetite nanoparticles also significantly had a marked bacteriostatic effect
on the studied microorganisms. This effect could be explained by mechanism of change
the polarization structure water of microorganisms by magnetite nanoparticles. It
was discovered that degree of expression of bacteriostatic action which induced
by magnetite nanoparticles had correlation with marks of reactions intensity of
FRLP. Maximum bacteriostatic effect on S.
aureus was expressed in second variant application of magnetite nanoparticles
where mechanism of sorption was more significant than action of the magnetic field.
On the contrary, maximum bacteriostatic effect on E. coli and K. pneumoniae was revealed in third variant, where time exposition of contact with microorganisms
nanoparticles and, consequently, action of a constant magnetic field was determinative.