Valledor, L., Rodríguez, R., Sánchez, P., Fraga, M., Berdasco, M., Hasbún, R., Rodríguez, J.L., Pacheco, J.C., García, I., Uribe, M., Ríos, D., Sánchez-Olate, M., Materán, M.E., Walter, C. and Canal, M.J. (2007) Propagation of Pinus Genotypes Regardless of Age. In: Jain, M. and Haggman, H., Eds., Protocols of Propagation for Woody Trees and Fruits, Springer, Dordrecht, 137-146.

has been cited by the following article:

• TITLE: Changes in Gene Expression in Needles and Stems of Pinus radiata Rootstock Plants of Different Ontogenic Age

  AUTHORS: Carolina Alvarez, Luis Valledor, Patricia Sáez, Rodrigo Hasbún, Manuel Sánchez-Olate, María Jesús Cañal, Darcy Ríos

  KEYWORDS: Plant Ageing, Gene Expression, Morphogenetic Competence, Pinus radiate

  JOURNAL NAME: American Journal of Plant Sciences, Vol.7 No.8, June 13, 2016

  ABSTRACT: A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot technique was performed in needles and stems of 1- and 3-year old Pinus radiata rootstock plants with a proved decrease in morphogenetic competence. Needles of one year old rootstock plants showed a higher number of up-regulated in genes mainly corresponding to photosynthesis and protein synthesis, degradation and modification, reflecting a higher number of active pathways in younger hedges, contrary to the older ones. Gene expression profiles found in stems are in agreement with those found in needles, indicating more active pathways in younger rootstock plants than in older ones. Several transcripts regulating transcription and translation were up-regulated in young competent tissues. Three-year-old stems presented an increase in the expression of an ethylene response factor, involved in plant organ senescence, indicating that pathways involved in senescence and ageing might inhibit the adventitious root formation, as in the older cuttings.