TITLE:
Modulation Possibilities of the Tissue Wettability, Marker of the Physical Modifications Produced by Senescence versus Photo Senescence at Tegument Level
AUTHORS:
Laurentiu Badescu, Carmen Elena Cotrutz, Raoul Lupusoru, Bogdan-Mihail Diaconescu, Manuela Ciocoiu, Magda Badescu
KEYWORDS:
Skin, Photoimmunology, Phototherapy
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.7 No.13,
November
19,
2014
ABSTRACT: We used an
experimental model involving white Westar rats (young and old) who were
subjected to precise doses of ultraviolet radiation (UV). The experiment aims
to examine the influence of epidermis potential protection factors in photo
senescence versus senescence. We have studied the in vivo effect of some natural polyphones extracted from black
grapes seeds and zinc aspartate and in
vitro effect of AED (deuterium depleted water). The substances were
administered by intragastric gavages, at two days intervals for two weeks prior
to UV irradiation and during the entire period of the experiment (28 days). In
the end, we determined the degree of hydrophilicity of the skin, in vitro, by measuring the contact angle
value, which was inversely proportional to the hydrophilicity of the tissue
(the angle formed at the contact between a liquid and a solid surface). Using
this method we found a large variability depending on the hydrophilicity of the
epidermis (abdomen and back), the age of the animal and UV/non UV irradiation.
The maintenance of the epidermis fragments in AED (for 6 hours) increased
significantly the tissue wet ability degree both in the young rats subject to
UV and in the senescent ones subject to UV (photo senescence).