TITLE:
Comparative Studies of Bacillus thuringiensis var. israelensis Metabolism in Different Concentrations of Cassava Flour Processing Waste Based Media
AUTHORS:
Samara Ernandes, Vanildo L. Del Bianchi, Iracema O. Moraes
KEYWORDS:
Bioinsecticides, Agroindustrial Wastes, Bacillus thuringiensis var. israelensis, Fermentation
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.12,
October
31,
2014
ABSTRACT: Techniques of production of enthomopatogenic bacteria are developed aiming to increase the productivity and to reduce
the costs of the fermentative process. Like this, it has been using agroindustrial wastes or by-products as nutrient sources in culture medium, having
been used, in this study, the manipueira, a by-product of the processing of the
cassava flour. Fermentations were performed in flasks of Erlenmeyer of 500 mL
containing 250 mL of culture media, conditioned in shaker at 180 r.p.m. and 28°C,
and the media were composed by manipueira, in concentrations that varied
between 400 and 1000 mL/L. The time of the process varied between 48 and 120
hours. They appraised the following parameters: cellular growth, the production
of spores, the reduction of organic matter (COD analysis) and the variation of
reduction sugar. Although there was a proportional cellular growth to the
manipueira concentration, the production of spores was similar in all the
cases, at the end of the process, in spite of the smallest speed of production
of the same ones in the highest concentrations. In relation to the variation of
COD, it has, also, a percentile minor of reduction in the highest
concentrations. In the analysis of variation of reduction sugars, the higher
concentrations are the ones that they present larger slowness in the reduction
of this.