TITLE:
Contribution to Confirmed & Synthesized on Mycorrhizae of Tuber indicum s.l. with Two Dominated & Subalpine Broadleaf Trees in Southwestern China
AUTHORS:
Xiaojuan Deng, Fuqiang Yu, Peigui Liu
KEYWORDS:
Host Preference, Populus yunnanensis, Quercus pannosa, Mycorrhizae, Truffle, Conservation & Plantation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.21,
October
28,
2014
ABSTRACT: The ascomata and
mycorrhizae of Tuber indicum s.l. were collected under the forest of
broad-leaf species Populus yunnanensis and Quercus pannosa in the field
respectively. The symbiotic relationships of both trees with T. indicum were examined and affirmed
based on morphology and ITS-rDNA sequences. These two mycorrhizal combinations
were successfully produced on artificially controlled substrates and cultural
condition. This is the first report of a mycorrhizal association and
synthesis between Chinese black truffles and poplars. A hyphal net covering the
mantle’s surface of the mycorrhizae was detected in both mycorrhizal
combinations. The mycorrhizal colonization of P. yunnanensis and Q. pannosa suggests that T. indicum s.l. has a broader host range and that
additional corresponding wood species would be used as candidates for the
cultivation of T. indicum. The
nuclear-ITS sequences of the mycorrhizae included in the phylogeny of the T. indicum complex revealed that the two
clades within the complex do not markedly differ with respect to their
preferences for host species or geographical origin. Our results help to
explain the wide distribution of both clades of the T. indicum complex. It would be more important for truffle conservation
and Chinese black truffle plantation development with these two dominated &
alpestrine Populus yunnanensis and Quercus pannosa at subalpine limestone
areas in China.