TITLE:
Amino Acid Composition of Tightly Bound Exopolymeric Substances Produced by Corrosion-Related Bacteria in Presence of Mild Steel
AUTHORS:
Mariia Boretska, Iaryna Datsenko, Olga Suslova, Olena Pareniuk, Olena Moshynets
KEYWORDS:
EPS, Stenotrophomonas maltophilia, Biofilm, Amino Acid Composition, Ion Exchange Chromatography, Mild Steel, Biocorrosion
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.12,
September
23,
2014
ABSTRACT: The composition of
tightly bound exopolymeric substances (EPS) obtained from biofilm and plan- ktonic
cell subpopulations of Stenotrophomonas
maltophilia 5426 UKM was analyzed to study an impact of mild steel presence
in the cultivation medium. The overall protein production was found to increase
in the presence of a steel coupon. Some amino acids such as lysine, valine,
iso-leucine, tyrosine and phenylalanine were found to be secreted in higher
amounts in the presence of mild steel, while its absence leads to an increase
of arginine, asparagine, serine, glutamine, proline, glycine, cysteine,
leucine, methionine production levels. Biofilm cells EPS contained more
arginine, glycine, cysteine, valine, methionine and leucine, comparing to EPS
of planktonic cells. The chang- es of tightly bound EPS aggregation around the
cells induced in the presence of steel coupons were revealed by transmission
electron microscopy. The results suggested the transition of S. maltophilia 5426 UKM cells from
planktonic to biofilm lifestyle to be a complex process involving more than a
single step.