TITLE:
In Vitro Induction of Polyploidy in Citrus reticulata Blanco
AUTHORS:
Doaa M. Abou Elyazid, Ali R. El-Shereif
KEYWORDS:
Colchicine, DNA, Mandarin, Polyploidy, Tetraploid
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.11,
May
21,
2014
ABSTRACT:
Possibility of
polyploidy induction by colchicine in “Balady” mandarins was investigated in vitro. Seeds were immersed in
different concentrations of colchicine solutions (0.01%, 0.05%, 0.1% and 0.2%)
for different durations (12, 24 and 48 hr), then cultured in vitro on MS
medium at half strength. Seed survival percentage decreased by increasing
colchicine concentration and duration of treatment showing the lowest
percentage at 0.2% for 48 hr. The highest DNA content was recorded at 0.2% for
24 hr. Stomata No. per unit area was decreased by colchicine treatments; moreover stomata length and width were studied. The
results indicated that colchicine treatment at 0.1% for 48 hr had the
highest tetraploid induction efficiency percentage.