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has been cited by the following article:

• TITLE: Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum

  AUTHORS: Tanzila Shahzadi, Zahid Anwar, Zafar Iqbal, Awais Anjum, Tahir Aqil, Bakhtawar  , Arroha Afzal, Muhammad Kamran, Sajid Mehmood, Muhammad Irshad

  KEYWORDS: Process Optimization; SSF; Co-Culture; T. viride; G. lucidum

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.5 No.5, April 2, 2014

  ABSTRACT: In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.