TITLE:
Optimum Protocol for Shoot Formation in karandafi Red Sorghum (Sorghum bicolor (L.) Moench) through Somatic Embryogenesis Using Mature Embryo
AUTHORS:
Lateefat Bolanle Hassan, Inuwa S. Usman, Mary D. Katung, Sanusi M. Bugaje
KEYWORDS:
Sorghum bicolor; Callus Induction; In-Vitro Regeneration
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.5,
March
14,
2014
ABSTRACT:
In this study, an optimum protocol for
shoot formation through somatic embryogenesis using mature embryo explants was
developed. Calli were initiated on Murashige and Skoog (MS) media supplemented
with varying concentration of 2,4-Dichlorophenoxyl acetic acid (2,4-D) ranging
from 1.5 mg/l-4.0 mg/l alone or in combination with 0.5 mg/l Kinetin (KN). Significance difference (p 0.05) was observed among
the different concentrations of hormone used for callus induction. The highest
percentage callus formation was obtained from the media fortified with 4 mg/l 2, 4-D for mature embryo obtained from imbibed seed while for the
preconditioned mature embryo, the media supplemented with 2 m/l 2,4-D + 0.5 mg/l kinetin (KN)
recorded more percentage callus formation compared to what was obtained from
the media supplemented with 2.5 mg/l2,4-D + 0.5 mg/l. More percentage shoot formation was obtained from the media supplemented
with 1 mg/l 6-benzylaminopurine
(BA). Average number of shoot per callus was also more in the media fortified
with 1 mg/l BA (2.25) but this was not significantly
different from what was obtained from the media fortified with 2 mg/l BA+ 0.1 mg/l 2,4-D at 5% level of
significance.