TITLE:
Effect of BA Treatments on Morphology and Physiology of Proliferated Shoots of Bambusa vulgaris Schrad. Ex Wendl in Temporary Immersion
AUTHORS:
Yudith García-Ramírez, Mallelyn Gonzales Gonzáles, Elisa Quiala Mendoza, Marisol Freire Seijo, Mariana La O Cárdenas, Leonardo Julio Moreno-Bermúdez, Ortelio Hurtado Ribalta
KEYWORDS:
Cytokinin; Bamboo; Hyperhydricity; Liquid Medium; Semi-Automation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.2,
January
24,
2014
ABSTRACT:
Axillary buds, collected from greenhouse-grown
plants of Bambusa vulgaris Schrad. ex
Wendl (B. vulgaris),
were incubated on a static
liquid culture medium, Murashige and
Skoog (MS) medium with 2% (w/v) sucrose, and supplemented with 12.0 μM
6-benzyladenine (BA). They were
transferred to a temporary immersion system (TIS) using liquid MS medium
supplemented with 0 (CK-free medium), 6.0, 12.0, 18.0 μM BA. The morphological and anatomical indicators were measured. The BA
influenced in vitro multiplication of B. vulgaris. The best results were
achieved in the SIT with a concentration of 6.0 μM of BA, which increased
the number of shoots (5.1 shoots/explant) in the absence of hyperhydric shoots.
Results demonstrated that the water content in the sprouts increased with 12.0
and 18.0 μM BA every four hours. Furthermore,
these high levels of BA contributed to a lower accumulation of phenolic
compounds and lignin content. The total chlorophyll significantly increased
when using 6.0 uM BA, but decreased both parameters with other treatments.
These results favor to increase the number of shoots/explants during in vitro multiplication. They will also
optimize the in vitro culture
conditions, leading to an improvement of in
vitro propagation methods for this species.