TITLE:
In Vitro Evaluation of Ozone Activity on Recent Clinically Isolated Bacterial Strains
AUTHORS:
P. Tordiglione, F. S. M. Araimo Morselli, I. Scarpa, G. Puggioni, C. Mancini, G. Rosa, A. Giordano
KEYWORDS:
Ozone; Bactericidal Activity; In Vitro; Media; Therapeutic Treatment
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.2,
January
21,
2014
ABSTRACT:
This study aims to evaluate
the cozone bactericidal activity in different suspension media (saline,
broth and whole blood) at different exposure times. Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, ESBLpositive Escherichia coli, MDR Pseudomonas aeruginosa were suspended in
different media. We used a bacterial concentration of 0.2 MF for all
experiments, as this concentration is consistent with the results of septic
shock blood experiments. We performed ozone insufflations in a “sealed
environment”. The total number of insufflations for each experiment ranged from
one to four. The gas concentration was maintained at 80 mcg/ml. We confirmed
the bactericidal activity of ozone on saline for all the bacterial strains.
Experiments in broth revealed no changes in the bacterial growth. Ozone is
primarily bactericidal against E. coli and
bacteriostatic on P. aeruginosa, S. aureus and E. faecalis on whole blood. This study confirms the bactericidal
efficacy of topical ozone applications and supports the need for further
evaluations of the therapeutic potential of major ozone autohemotherapy. The
results in E. coli promote further
investigations of ozone activity on other Enterobacteriaceae and its potential
use in the treatment of urinary infections. In general, these results suggest
that ozone-therapy might be an alternative therapy to overcome antibiotic
resistance.