TITLE:
Effect of 24-Epibrassinolide on Growth of in Vitro Shoot Tips of Different Yam (Dioscorea Spp.) Species
AUTHORS:
Isabelle Engelmann-Sylvestre, Florent Engelmann
KEYWORDS:
Yam; Dioscorea Spp.; 24-Epibrassinolide; Oxidation; Shoot Tip; In Vitro Culture; Cryopreservation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.11,
November
22,
2013
ABSTRACT:
In this work we compared the effect of the growth regulator content of
the culture medium on the growth of in
vitro shoot tips of five yam accessions belonging to four yam species (one Dioscorea alata, one D. rotundata, one D. cayenensis and two D.
trifida). Medium S contained 0.6 μM benzyl adenine, 1.07 μM naphthalene
acetic acid and 0.23 μM gibberellic acid while medium EBR contained 0.23 μM
gibberellic acid and 0.1 μM 24-epibrassinolide.
After 2 months of culture, oxidation level was significantly reduced on medium
EBR compared to medium S for four of the five accessions tested. By contrast,
medium EBR did not have any positive effect on shoot length since length of
shoots produced after 2 months of culture on medium S and EBR were similar,
except with accession 3-45T, for which shoot length was shorter on medium S
compared to medium EBR. These results underline the potential of 24-epibrassinolide
to reduce oxidation phenomena during in vitro culture and call for
its utilization for regeneration of cryopreserved yam shoot tips, which is
often impeded by oxidation phenomena.