TITLE:
In Vitro Chromosomal Aberration Frequency by Electrofishing on Poecilia latipinna (Sailfin Molly) Fishes in Southern of Iraq
AUTHORS:
Mohammed A. Abd Ali, Mohammed H. Mohammed, Marwa K. Sadeq
KEYWORDS:
Electrofishing, Chromosomal Aberration, Poecilia latipinna (Sailfin Molly)
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.8 No.2,
April
26,
2018
ABSTRACT: The present studies describe Chromosomal aberration effects of electrofishing, which were
evaluated on Poecilia latipinna, located in Shat Al-Arab river in Al-garmma city (south of Iraq). The electrofishing
derive used in work is simulated to that used in the commercial fishing. The
apparatus generates voltage ranged from 40 to 280 volts. Nine bearers of Poecilia latipinna sailfin molly fish in
chromosomal analysis were divided into three treatments. The first were a
control, the fishes of the second were exposed to 110
volts (10 seconds), and final groups were exposed to 110 volts (15 seconds). Mitotic index of the electrofishing with
a control for each group decreased with increasing exposed time in somatic cell
kidney tissue of Poecilia latipinna. The chromosome aberration analysis revealed a
significant increase in the most frequent aberration per 150 metaphase in
analyzed groups (1.33 in T1 groups, 39.33 in T2 groups) was chromosome break, fragment, range chromosome, Sticky
chromosome mean, were higher in comparison to non exposed electrical shock
fishing groups (control groups T1). At the same time, it showed a higher positive correlation of total chromosome aberration frequencies
between T1 and T2 groups, while, all fishes died in T3 groups. According to our results, we represented the first record in Iraq.