TITLE:
Regeneration of in Vitro Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos
AUTHORS:
A. R. Zuraida, Kumeran G. Sentoor, N. Ahmad, Farhanah M. S. Syahirah, Nazreena O. Ayu
KEYWORDS:
Hormone Manipulation, Plant Growth Regulator, MATAG Coconut, Zygotic Embryo, Callus Induction
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.3,
February
4,
2017
ABSTRACT: Zygotic embryos tissues have
found to be more responsive explants for clonal propagation of coconut. In the
present study, the feasibility of using zygotic embryos explants for clonal
propagation of a local coconut variety MATAG F2 was assessed. Callus was
induced by incorporating of cytokinin and auxin into the medium. The sliced
embryos explants were immersed in 1 M maltose for 60 mins, then with 0.05 M
maltose for 1 min and followed by 0.01 M maltose for 5 mins was the best for
prevention of phenolic compounds excretion. The callus formation depended on
the concentration of 2,4-D in the media and the best effect was observed with
the high level (2,4-D and BAP) tested. Attempts at inducing multiple shoot from
the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure.