TITLE:
In Vitro Anticancer Assessment of Annickia chlorantha (Oliv.) Setten & Maas Stem (Annonaceae) Bark from Democratic Republic of Congo
AUTHORS:
Damien S. Tshibangu, Selvaraj Divakar, Muthiah Ramanathan, Govindarajan Syamala, Koto-te-Nyiwa Ngbolua, Jean Chrysostome V. Mudogo, Dinangayi D. Tshilanda, Nicole M. Misengabu, Pius T. Mpiana
KEYWORDS:
Annickia chlorantha, Cancer, MCF-7 Cell Lines, PC-3 Cell Lines, Anti-Proliferative Compounds
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.4,
April
7,
2016
ABSTRACT: In
this study, we evaluated the anti-cancer property of five bark extracts and the
isolates from chloroform and ethyl acetate of Annickia chlorantha by the tetrazolium salt method (MTT method).
The anti-cancer activity was performed on human prostate cancer cell lines PC-3
and hormone-dependent breast cancer cell lines MCF-7. Results indicated that
the two isolates displayed interesting cytotoxicity towards MCF-7 cell lines
with CC50 of 3.84 CC50/mL and 4.87 CC50/mL for
chloroform and ethyl acetate respectively; while the total bark extracts showed
CC50 of 24.33 CC50/mL, 36.49 CC50/ mL and
73.52 CC50/mL for chloroform, ethyl
acetate and methanol extracts respectively. By the other hand on PC-3, the CC50 of the isolates were higher than the one on MCF-7, more than 10 CC50/mL
for both chloroform and ethyl acetate isolates and 49.14 CC50/mL,
77.33 CC50/mL, 89.38 CC50/mL and 92.37 CC50/mL,
respectively for chloroform, ethyl acetate and methanol soluble extracts. From
this study, we identified that the two isolates had anti-cancer properties
against MCF-7 cell lines.