TITLE:
Isolation and expression characterization of CBF2 in vitis amurensis with stress
AUTHORS:
Chang Dong, Jianmin Tao, Meng Zhang, Yang Qin, Zhiying Yu, Bailin Wang, Binhua Cai, Zhen Zhang
KEYWORDS:
vitis amurensis; Stress; CBF2; Expression
JOURNAL NAME:
Agricultural Sciences,
Vol.4 No.9,
August
27,
2013
ABSTRACT:
The
transcription factor VaCBF2, which interacts with C-repeat/DRE and its promoter,
was isolated from Vitis amurensis. The
VaCBF2 amino acid sequence contained a conserved AP2 domain of 56 amino acids and
a potential nuclear localization sequence. The sequence of VaCBF2 showed a high
level of homology with other CBF2 family members. Phylogenetic analysis showed
that the amino acid sequences may be CBF2 proteins with evolutionary
relationship. Quantitative reverse-transcription polymerase chain reaction
analysis indicated that the expression of
VaCBF2 gene in tissues (roots, stems, leaves, and petioles) was induced
by low temperature, high salinity, and application of abscisic acid and
salicylic acid in a time-dependent manner but to different extents in the
cold-hardy V. amurensis and the less
cold-hardy Vitis vinifera. The
presence of cis-elements such as MYC
and ABRE in VaCBF2 promoter further confirmed that this promoter was a
component of the CBF transduction pathway involved in plant response to
multiple stresses.