TITLE:
Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS
AUTHORS:
Jong-Shik Kim
KEYWORDS:
Polychlorinated Biphenyl (PCB); Carvone; Rhodococcus; Protein Expression
JOURNAL NAME:
Journal of Agricultural Chemistry and Environment,
Vol.2 No.3,
August
9,
2013
ABSTRACT: Protein expression patterns in the polychlorinated biphenyl (PCB)-degrading Rhodococcussp. strain ACS were examined following growth on two substrates capable of inducing the enantioselective biotransformation of PCBs via different degradation pathways. Eleven
inducible proteins were identified by SDS-PAGE and characterized by LC-MS/MS. Four of the peptides, a spore coat protein, an extracellular serine protease, a spoVP, and a molecular chaperonin from Bacillus subtilis, were identified as being unique to biphenyl-induced cells, whereas anextracellular serine protease from B. subtilis was identified as being unique to carvone-induced cells.
None of the peptides identified had sequences that corresponded to known dioxygenases or other PCB-degrading enzymes of this Gram- positive bacterium, suggesting that the identified induced proteins may be involved in either PCB degradation or adaptive responses that protect cells from toxicity.