TITLE:
Determination of okadaic acid related toxins from shellfish (sinonovacula constricta) by high performance liquid chromatography tandem mass spectrometry
AUTHORS:
Hai-qi Zhang, Weicheng Liu, Xin He, Li-jun Liang, Wenyong Ding, Zhong-yang He
KEYWORDS:
Sinonovacula Constricta; High Performance Liquid Chromatography-Tandem Mass Spectrometry; Okadaic Acid; Dinophysistoxins-1
JOURNAL NAME:
Agricultural Sciences,
Vol.4 No.5B,
July
12,
2013
ABSTRACT:
Consumption
of shellfish contaminated with algal toxins
produced by marine dinoflagellates can lead to diarrhetic shellfish poisoning
(DSP). It was therefore essential that there are analytical techniques to
identify and quantify DSP toxins in
shellfish. This new methodology could facilitate DSP monitoring and
create a means of rapidly responding to
incidents threatening public health. In the last years there were
different analytical methods for DSP, such as mouse bioassay and LC-FLD. With
the development of instrument, Liquid chromatography-mass spectrometry was
substituted for other analytical methods with its good sensitivity and
selectivity and without derivatization for the determination of DSP. In this
report, a high performance liquid chromatogra-phytandem mass spectrometric（HPLC-MS/MS）method was developed
for the simultaneous determination of okadaic acid (OA) and dinophysistoxins(DTX1)
in Sinonovacula constricta.
Optimization of pretreatment experiment was carried out to maximize recoveries
and the effectiveness. The analytes were determined under multi-reactions
monitoring (MRM) scan type with tandem mass analyzer using negative ion
electrospray ionization (-ESI) mode .Finally, the detection and identification
of OA and DTX-1 were based upon their retention times (RT) and the
fragmentation patterns of their mass spectra. The method of LOQ for the two
poisons was 0.02 mg·kg-1.The
real sample test showed that this method could be used for sensitive, fast, and
accurate determination of the two diarrheic shellfish poisons in shellfish.