TITLE:
Analysis of N-Nitrosamine Migration from Rubber Teats and Soothers
AUTHORS:
Motoh Mutsuga, Miku Yamaguchi, Yoko Kawamura
KEYWORDS:
N-Nitrosamine; N-Nitrosatable Substances; Secondary Amine; Rubber Teat and Soother; GC-MS
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.6,
June
20,
2013
ABSTRACT:
A testing method for N-nitrosamines
and N-nitrosatable substances in
rubber teats and soothers was modified. N-Nitrosamines
are generally analyzed using either a nitrogen chemiluminescence detector (NCD)
or a thermal energy analyzer (TEA). However, because few testing
laboratories are equipped with these devices, it is difficult to conduct these
tests. Therefore, an analysis method for N-nitrosamines
using the more widespread gas chromatography-mass spectrometry
(GC-MS) method was improved. In addition, EN 12868 was used to prepare the test
solutions because of its worldwide use and compliance with EU regulations.
Using GC-MS, EN 12868 method targeting ten kinds of N-nitrosamines was modified. The determination limits of the method were 1.0 -1.5 μg/kg for N-nitrosamines and 4 -6 μg/kg for N-nitrosatable
substances. Quantification was possible at 1/5 or less and 1/15 or less,
respectively, of the regulation values listed in EU Directive
93/11/EEC. In terms of application, there were no problems with the selectivity
of the detector. The recoveries were 58% -109% for N-nitrosamines and 59% -102%
for N-nitrosatable substances.
Screening and verification were possible by measuring the amount of secondary
amines in the boiled solution and migration solution.