TITLE:
Overexpression of the recombinant xyloglucanase sp-Xeg from Penicillium canescens accelerates growth and rooting of transgenic aspen plants
AUTHORS:
Vidyagina Elena, Kovalitskaya Yulia, Loginov Dmitry, Koroleva Olga, Shestibratov Konstantin
KEYWORDS:
Populus Tremula; Activity of Xyloglucanase; Pentosans; Efficiency of Rooting
JOURNAL NAME:
Natural Science,
Vol.5 No.6A,
June
18,
2013
ABSTRACT:
Analysis of the
properties of transgenic aspen clones with recombinant gene xyloglucanase sp-Xeg from fungi Penicillium
canescens showed the presence of complex modifications both in the
wood and the phenotype of plants. Biometric analysis revealed an increase in
the height of transgenic plants as compared to control plants. Increasing in
the height of the shoot of 24.8%, 25% and 26% was observed for lines
PtXIVXeg1a, PtXVXeg1a, PtXVXeg1b, respectively. Also there was an increase in
the number of internodes in some transgenic clones. For the first time we
showed the change in plants rhizogenesis with the recombinant gene xyloglucanase.
In 10 of the 25 lines the rooting efficiency in vitro exceeded the control value. The maximum value of the
rhizogenesis was fixed for line PtXVXeg1a (2.5 times higher than the control
value). The mass of the root system for 6 of the 25 clones in the greenhouse
was higher by 20% than the control value. The pentosan content decrease was
also detected in all wood samples of transgenic plants. The obtained data of
xyloglucanase activity and pentosan content generally correlated with phenotypic
modifications.