TITLE:
Co-Orelation of Histological Dating and Glycogen Content by Histochemical Stain during Various Phases of Menstrual Cycle in Primary Infertility
AUTHORS:
Anshu Gupta, S. K. Mathur, Anjali Gupta
KEYWORDS:
Glycogen; Endometrium; Primary Infertility
JOURNAL NAME:
Open Journal of Pathology,
Vol.3 No.2,
April
25,
2013
ABSTRACT:
Glycogen and its
products of metabolism are considered to be the most important and direct source
of nutrient for the early conceptus. The premenstrual biopsies were taken from 100
women with primary infertility whose male partners had normal semenograms. The biopsies
were then subjected to Haematoxylin and Eosin (H & E) and Periodic Acid Schiff (PAS) staining with and
without diastase to study histological and histochemical changes particularly glycogen
content in the endometrium respectively. Histolomorphologic spectrum of endometrium
on H & E stained sections revealed early proliferative
phase (4%), late proliferative phase (7%) and early secretory phase (7%), mid secretory
phase (33%) and late secretory phase (49%). PAS staining with or without diastase
for glycogen was performed and glycogen
content, as demonstrated by magenta color, was graded from + to ++++. Glycogen content was observed to be “+” to “++” in proliferative phase, where as in secretory
phase, it varied from “++” to “+++”. Glycogen was confirmed by treatment with
diastase enzyme, after that PAS stain gave negative staining, i.e. loss of magenta color as glycogen was digested by diastase. Glycogen deficiency
was observed in 24.7% of secretory phase endometrium of infertile women. Depletion
of glycogen results in inadequate preparation of endometrium at the time of implantation, and hence, may be one of the causes of
infertility.