TITLE:
Flash microbiocide: A Rapid and Economic Method for Determination of MBC and MFC
AUTHORS:
Camila Hernandes, Juliana da Silva Coppede, Bianca Waléria Bertoni, Suzelei de Castro França, Ana Maria Soares Pereira
KEYWORDS:
Antimicrobial Activity; Minimal Inhibitory Concentration; Minimal Bactericidal Concentration; Minimal Fungicidal Concentration; Methodology
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.4,
April
17,
2013
ABSTRACT:
Although
nowadays there are methods for determining the Minimal Bactericidal Concentration
and Minimal Fungicidal Concentration, it is indispensable thatthe establishment of
innovative methodologiescould be more practical and cheaper. The new
methodology Flash microbiocide is an assay in which one aliquot from
96 well plate of Minimal Inhibitory Concentration test is transferred to
another plate containing different culture medium. The correspondence with the
reference methods described in the National
Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was
achieved, denoting the efficiency of
this fast and simple method.