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has been cited by the following article:

• TITLE: Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated

  AUTHORS: Fika Ayu Safitri, Mohammad Ubaidillah, Miswar  , Kyung-Min Kim

  KEYWORDS: AtBI-1; Agrobacterium-Mediated; GFP; Transformation; Rice

  JOURNAL NAME: American Journal of Plant Sciences, Vol.4 No.4, April 17, 2013

  ABSTRACT: The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice (“Ilmi”) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.