TITLE:
The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana
AUTHORS:
María I. Vaquero-Sedas, Miguel A. Vega-Palas
KEYWORDS:
Arabidopsis thaliana; telomeres; ITSs; euchromatin; heterochromatin
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.2 No.3,
July
16,
2012
ABSTRACT: We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some concerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and interpretation of results.