TITLE:
Effects of Cornus Officinal Is Total Glycoside on Cardiomyocyte Apoptosis and Calcium Overload after Hypoxia/Reoxygenation Injury in Rats
AUTHORS:
Ke Chen, Liudan Chen, Qihui Huang, Aizhen Pan, Kefang Chen, Xiangping Hou, Xiaojin Xue, Jianjun Li
KEYWORDS:
Cornus officinalis Total Glycoside, Hypoxia/Reoxygenation, Cardiomyocyte Apoptosis, Calcium Overload
JOURNAL NAME:
Chinese Medicine,
Vol.10 No.1,
January
30,
2019
ABSTRACT:
Objective: To
investigate the mechanism of Cornus
officinalis Total Glycosides (COTG) on myocardial protection, by studying
effects of COTG on cardiomyocyte apoptosis induced by hypoxia/reoxygenation and
calcium concentration in rats. Methods: The myocardial cells of born
1-3d SD rats were isolated by enzyme digestion, cultured for 3 days. Cells were
divided into five groups: Control group, H/R group, Cornus officinalis Total Glycosides low-dose group (LDG), Cornus officinalis Total Glycosides middle-dose
group (MDG) and Cornus officinalis Total Glycosides high-dose group (HDG). Three drug groups were pretreated with
different doses of Cornus officinalis Total Glycosides before hypoxia/reoxygenation treatment. The apoptotic rate was
determined by flow cytometry assay, the intracellular free calcium
concentration was examined by flow cytometry, and the ultrastructure of
myocardial cells was observed under transmission electron microscope. Results: The results revealed that Cornus
officinalis Total Glycosides pretreatment decreased apoptosis rate, but the
effect of lower dosage is not significant. Furthermore, Cornus officinalis Total Glycosides can attenuate mitochondrial
calcium overload, improve mitochondrial morphology and inhibit cardiomyocyte
apoptosis caused by H/R. Conclusion: Cornus
officinalis Total Glycosides pretreatment can inhibit cardiomyocyte
apoptosis and calcium overload during H/R injury. However, the underlying
mechanisms require us to further study.