TITLE:
Study on Dormancy Mechanism and Breaking Dormancy Method of Viburnum sargentii Seeds
AUTHORS:
Qian Zhang, Ying Li, Xia Sun, Shiyan Xing, Wei Cong, Xuejun Liu
KEYWORDS:
Viburnum sargentii, Dormancy Mechanism, Endogenous Hormones, Breaking Dormancy
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.10 No.1,
January
14,
2019
ABSTRACT: Under
untreated conditions, the germination rate of Viburnum sargentii seeds
is very low. By exploring the relationship between the dynamic changes of
physicochemical indicators and endogenous hormones and seed germination during
the dormancy and germination of Viburnum sargentii seeds,
the mechanism of seed germination of Viburnum sargentii was
determined, which provided a theoretical basis for its extensive promotion and
development. The contents of soluble sugar and soluble starch were determined
by anthrone colorimetry. The soluble protein was determined
by Coomassie brilliant blue G-250 staining. The activity of polyphenol oxidase
(PPO) was determined by NBT reduction method. The peroxidase (POD) was
determined by guaiacol method. And the endogenous hormones ZA, IAA, ABA, GA3 were determined by liquid chromatography. Results show: 1) Under natural
conditions, the seeds of Viburnum sargentii are
difficult to germinate. 2) Under sand storage for 8 months, the germination
rate of untreated seeds was 33%. Puncture and peeling treatment could
significantly increase the germination rate, and the germination rate of seeds
treated with puncture was 92%, and that treated with peel was 98%. 3) Seed
germination was accompanied by the decrease in macromolecular substances such
as soluble sugar, soluble starch, and soluble protein. 4) The dynamic changes
of hormones during seed germination conform to the hypothesis of “three
factors”. 5) The treatment of puncture and peeling increased the content of
endogenous hormones promoting germination, decreased the endogenous hormones
inhibiting seed germination, and increased the ratio of (IAA + GA + ZR)/ABA or
GA/ABA. The seeds of Viburnum sargentii have
obvious dormancy characteristics. Under the condition of sand storage, both
pricking and peeling treatment can effectively promote the process of breaking
dormancy, and the effect of peeling treatment is better.