TITLE:
Effect and Tolerability of the Combined Plant Extract (PAC) in Treatment of Acne Vulgaris
AUTHORS:
Haesun Park, Sujung Woo, Jinsook Hyun, Hyeonji Kim, Jiyoung You, Hyunju Woo, Eunsun Jung, Kwangseon Jung, Myungok Kim
KEYWORDS:
Anti-Microbial Activity, Acne, Anti-Inflammatory, Clinical Study, Sebum
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.8 No.2,
June
21,
2018
ABSTRACT: This
study was undertaken to identify a new combined plant extract (PAC) intended to
treat in patients with acne vulgaris. We detected strong antibacterial
activities of Pinus densiflora leaf
extract (LP) against Propionibacterium
acnes (P. acnes) and Staphylococcus epidermidis (S. epidermidis) and anti-inflammatory
effect of Artemisia annua L. extract
(AA). To develop more effective agents in
multiple targeting of the physiopathology of acne, a combination of three plant
extracts (PAC), Pinus densiflora leaf
extract (LP), Artemisia annua L.
extract (AA), and Citrus junos fruit
juice (FC), indicated anti-proliferative effect on P. acnes-induced HaCaT cells proliferation, was prepared mixing at
ratio 2:1:1. PAC with MIC values of 2.25 mg/ml and 0.75 mg/ml against P. acnes and S. epidermidis was more active than LP (2.50 mg/ml and 1.00 mg/ml),
was the most active of three extracts. PAC also showed more effective
anti-inflammatory activity than a single plant extract in P. acnes-treated NF, HaCaT cells and LPS-treated Raw 264.7
cells. And then, we evaluated sebosuppressive effect of PAC on differentiation
and lipogenesis of sebocytes. Results showed that PAC reduced sebum production
and IGF-1-stimulated proliferation on sebocytes. In addition, PAC (final 2%)
applied in a test product clinically decreased skin oil content, sebum output
level, and the number of closed comedones and open comedones in a period of 4
weeks. The results of the studies demonstrate that PAC is clinically effective
with anti-bacterial, anti-inflammatory, sebum protective properties for acne
prone skin. These results suggest that in patients with acne, PAC can provide
adjunctive care by targeting pathological factors including bacterial
proliferation, inflammation and sebum production.