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https://doi.org/10.3390/pathogens3030667

has been cited by the following article:

• TITLE: Inhibition of Acinetobacter baumannii Biofilm Formation by Methanolic Extract of Nothoscordum bivalve

  AUTHORS: Hernández-Marín David Alejandro, Muñoz-Ortega Martín Humberto, Guevara-Lara Fidel, Gúzman-Lucio Marco Antonio, Sánchez-García Eduardo

  KEYWORDS: Biofilm, Sub-Lethal, Nothoscordum bivalve, Acinetobacter baumannii

  JOURNAL NAME: Advances in Microbiology, Vol.8 No.5, May 31, 2018

  ABSTRACT: Acinetobacter baumannii is one of the most prevalent pathogens in nosocomial infections and has been cause of concern in recent years because, it has presented multiresistance to antibiotics and besides can form biofilms on biotic and abiotic surfaces like tissues and medical devices. Therefore, the search for new alternatives of natural origin to inhibit biofilm formation is being conducted. In the present investigation, sub-lethal concentrations (5.61 mg/mL, 3.74 mg/mL and 1.87 mg/mL) of methanolic extract of Nothoscordum bivalve were evaluated, showing biofilm formation inhibition up to 40.8%, in one nosocomial isolated of A. baumannii by the microtiter biofilm formation assay using crystal violet. On the other hand, the concentrations of 5.61 mg/mL and 3.74 mg/mL, caused an overexpression (up to 15.4 times) in the genes involved in the formation of biofilm (abaI, bap and csuE); due to this, the interaction of the extract with the bacteria was analyzed by scanning electron microscopy (SEM) and cellular damage was observed in the structure and stability of biofilm.