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has been cited by the following article:

• TITLE: In Search of Regulators of LeSPL-CNR by South-Western Blotting and Yeast One-Hybrid Library Screening System

  AUTHORS: Xiaohong Wang, Bishun Ye, Ying Wang, Ting Zhou, Tongfei Lai

  KEYWORDS: Solanum lycopersicum, LeSPL-CNR, 286 bp Region, South-Western Blotting, Yeast One-Hybrid

  JOURNAL NAME: American Journal of Plant Sciences, Vol.9 No.5, April 20, 2018

  ABSTRACT: LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.