TITLE:
Isolation and Functional Analysis of the Regulation of Branching by Isopentenyl Transferase Gene CmIPT1 in Chrysanthemum morifolium cv. ‘Jinba’
AUTHORS:
Abdurazak Ishak, Lili Dong, Huang Rong, Shuiming Zhang, Liangjun Zhao
KEYWORDS:
Chrysanthemum, Cytokinin, CmIPT1, Shoot Branching
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.8 No.2,
April
13,
2018
ABSTRACT: Today’s chrysanthemums are highly evolved flowering plants and they are considered
as one of the most important ornamental cut flowers. In this research an isopentenyl
transferase gene named CmIPT1 was isolated from Chrysanthemum
morifolium cv. ‘Jinba’ using RACE and RT-PCR methods. The full cDNA sequence of CmIPT1 was 873 bp which encoded a deduced
protein of 290 amino acids. It contained
GxxGxGKS which is a conserved sequence of the typical domain of IPT family. The
phylogenetic tree analysis of CmIPT1
in Chrysanthemum morifolium cv. ‘Jinba’ shows that it has the closest relationship with CcIPT1 in Cynara cardunculus var. scolymus. Expression of CmIPT1 was higher in stems and apex,
whereas it was lower in leaves and roots. And the overexpression of CmIPT1 obviously increased the number of
rosette branches in Arabidopsis. Here, in our study, we showed that CmIPT1 is a positive regulator of branch development
in Chrysanthemum and may play a key
role in regulating lateral branch formation of Chrysanthemum plants.