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Suzuki, T., Kakizaki, H., Ikeda, M. and Matsumiya, M. (2014) Molecular Cloning of a Novel Chitinase Gene from Blue Shark (Prionace glauca; Chondrichthyes) Stomach. Journal of Chitin and Chitosan Science, 2, 143-148.
https://doi.org/10.1166/jcc.2014.1050

has been cited by the following article:

  • TITLE: Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis

    AUTHORS: Miku Watanabe, Hiromi Kakizaki, Momo Kanai, Satoshi Kawashima, Kaneyuki Hamaguchi, Hiroki Mizuno, Tsubasa Ueno, Chiaki Yasukawa, Ryuji Agata, Mana Ikeda, Hideto Fukushima, Mitsuhiro Ueda, Masahiro Matsumiya

    KEYWORDS: Chitinase, Distribution, Chondrichthyes, cDNA Cloning, Phylogenetic Analysis

    JOURNAL NAME: Open Journal of Marine Science, Vol.8 No.1, January 31, 2018

    ABSTRACT: We have previously reported the presence of three types of chitinase (acidic fish chitinase-1: AFCase-1, acidic fish chitinase-2: AFCase-2, fish chitinase-3: FCase-3) in Actinopterygii. In the present research, we report the identification of the novel chitinase genes HjChi (ORF: 1380 bp) and DkChi (ORF: 1440 bp) from the stomach of Chondrichthyes, Japanese bullhead shark (Heterodontus japonicas) and Kwangtung skate (Dipturus kwangtungensis), respectively. Organ-specific expression analysis identified the stomach-specific expression of HjChi, whereas DkChi was expressed widely in all organs. Chitinase activity was measured using pNP-(GlcNAc)n (n = 2, 3) as a substrate and β-N-acetylhexosaminidase (Hex) activity was measured using pNPGlcNAc. Relatively high values of chitinase activity were observed in the stomach, spleen, and gonads of the Japanese bullhead shark, H. japonicas , compared with that observed in the stomach of the Kwangtung skate D. kwangtungensis . However, Hex activity was detected throughout the body of both species. The optimal pH of chitinase in both the Japanese bullhead shark, H. japonicas, and the Kwangtung skate, D. kwangtungensis, were 3.5 - 5.5 and 3.5 - 4.0, respectively, and 4.0 for Hex in both species. Phylogenetic analysis revealed that Chondrichthyes chitinase forms a unique group (Chondrichthyes chitinase). These results suggested that the possibility of the formation of chitinase groups for each class in the phylogenetic analysis based on the observation of class-specific chitinase.