TITLE:
Impact of Some Antibiotics on Bacteria Isolated from Appendices in Kirkuk Province, Iraq
AUTHORS:
Ayoub A. Bazzaz, Diyari A. K. Lor, Najdat B. Mahdi
KEYWORDS:
Bacteria, Antibiotics, Appendix, Appendectomy, Iraq
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.9 No.1,
January
19,
2018
ABSTRACT: A
bacteriological study of 50 appendectomy cases for patients diagnosed by
physicians to be appendicitis, within Kirkuk province was carried out to
isolate the habitat bacteria of appendix and to assess the impact of some
antibiotics on isolated bacterial species from the appendices. The age of
patients ranged between 3 - 45 year
old (21 male and 29 female). The culture results showed 94% single isolate with
2% negative growth and 4% mixed growth were obtained. The gram positive bacteria
isolate formed (11.77%) less than gram negative (88.23%). The total isolates
represented 51 included Escherichia
coli 34 (66.66%), followed by Enterococcus
faecalis 3 (5.89%), Klebsiella
pneumonia 3 (5.89%), Citrobacter
youngae 2 (3.92%), Raultella
terrigena 2 (3.92%), Pseudomonas
aeruginosa 2 (3.92%), Enterrobacter
cloacae 1 (1.96%), Serratia
fonticola 1 (1.96%), Entercoccus
faecium 1 (1.92%), Staphylococcus
epidermides 1 (1.96%) and Staphylococcus
xylosus 1 (1.96%). The Staphylococcus
xylosus was
the first time to be isolated in Kirkuk city while both of Raultella
terrigena and Citrobacter
youngae were
isolated for the first time in appendix samples. Only 16 various antibiotics
were tested against Enterobacteriaceae and Pseudomonas
aeruginosa while
12 and 7 against Staphylococcus
sp. and Enterococcus
sp.,
respectively. The Imipenem and Amikacin were found to be most effective
antibiotics against all Enterobacteriaceae isolates and Pseudomonas
aeruginosa while
Ampicillin, Piperacillin, Ceftazidime, Augmentin, and Cephalothin were
ineffective against all isolated bacteria. The Penicillin and Vancomycin
were effective against gram positive bacteria.