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Krishnan, K.R., James, F. and Mohan, A. (2016) Isolation and Characterization of n-Hexadecanoic Acid from Canthium parviflorum Leaves. Journal of Chemical and Pharmaceutical Research, 8, 614-617.

has been cited by the following article:

  • TITLE: Biological Control of Pyricularia oryzae Using Antifungal Compounds Produced by Aspergillus niger

    AUTHORS: Ali Abdulameer Idan, Kamaruzaman Sijam, Jugah Kadir, Tavga Sulaiman Rashid, Hayman Kakakhan Awla, Wael Alsultan

    KEYWORDS: Blast Disease, Aspergillus sp., Pyricularia oryzae, Antifungal Compounds, GC-MS

    JOURNAL NAME: American Journal of Plant Sciences, Vol.8 No.10, September 15, 2017

    ABSTRACT: Aspergillus spp. has been widely found as useful microorganism in biotechnology. They have a high ability in the production of secondary metabolites. Therefore, isolates of Aspergillus were isolated from healthy rice field located in Selangor State/Malaysia. The obtained strain (UPMZ01) was conducted against Pyricularia oryzea by applying dual culture and culture filtrate technique. The antagonism of strain UPMZ01 in the dual culture was 81.326% inhibition percentage against P. oryzae given the optimum inhibitory percentage 100% at all concentration of secondary metabolites aged 14 days. The isolate (UPMZ01) was identified as Aspergillus niger with accession number (KY698415). The environment factors such as pH and temperature influencing on production of secondary metabolites. The results were shown that pH at level 5.0 and temperature between 21℃to 29℃is the optimum condition for A. niger to produce efficient antifungal metabolites which given 100% PIGR against blast pathogen. The secondary metabolites compounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS). Fifteen compounds were recognized as major compounds which may have the possibility of possessing antifungal characteristics. Most of identified compounds are Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol, Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural.