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Article citations


Focke, M., et al. (2010) Lab-on-a-Foil: Microfluidics on Thin and Flexible Films. Lab on a Chip, 10, 1365-1386.

has been cited by the following article:

  • TITLE: Microfluidic Extreme PCR:

    AUTHORS: James M. Trauba, Carl T. Wittwer

    KEYWORDS: PCR, Microfluidics, Diagnostics, Point-of-Care

    JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.10 No.5, May 18, 2017

    ABSTRACT: Point-of-care diagnostic testing using PCR requires a device that is fast, economical, and practical. Sub-minute amplification has been demonstrated using high concentrations of primers and polymerase in glass capillaries, but its platform is limited to research use. A system using heated copper blocks to clamp a microfluidic flow-through PCR card fabricated from thin film polycarbonate was modeled, fabricated, and tested. Models show that fluid flowing through a thin-film device clamped between temperature-controlled copper blocks equilibrates to a temperature change in 250 milliseconds. A 2-step, 35 cycle PCR with 1.06 second cycles specifically amplified a 69-base pair fragment from a 450-base pair synthetic DNA template of random sequence with the same performance as the glass capillary system. This system demonstrates the feasibility of