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Zheng, Y.M., Zheng, S.J., Wang, Y., Lu, A.X. and Shing Ho, W. (2015) Anti-Oxidant and Anti-Cancer Activities of Angelica dahurica extract via Induction of Apoptosis in Colon Cancer Cells. Phytomedicine, 23, 1267-1274.
http://dx.doi.org/10.1016/j.phymed.2015.11.008

has been cited by the following article:

  • TITLE: Research of Anti-Cancer Components in Traditional Chinese Medicine on Hollow Fibre Cell Fishing and Hollow Fibre Liquid Phase Microextraction

    AUTHORS: Feixue Li, Xuan Chen, Yanli Xu, Xiaohong Bai

    KEYWORDS: Hollow Fibre Cell Fishing, Liquid Phase Microextraction, Identification, Quantification, Cancer Cell, Activity Screening

    JOURNAL NAME: American Journal of Analytical Chemistry, Vol.7 No.10, October 13, 2016

    ABSTRACT: Hollow fibre cell fishing with HPLC (HFCF-HPLC) based on the human ovarian cancer cell line SKOV-3, human renal tubular cell line ACHN or hepatoma cell line HepG-2 was employed to screen active groups of coumarin and volatile oil in Radix angelicae sinensis, Radix angelicae dahuricae and Fructus citri sarcodactylis. Simultaneously, hollow fibre liquid phase microextraction with HPLC (HFLPME-HPLC) was conducted to enrich and determine the contents of active components in the same sample solution. Before application, for HFCF-HPLC, cells growth states and survival rates on the fibre, effect of ethanol concentration in the extract of samples on cell survival rates, non-specific binding between fibre active centres and the target components, positive and negative controls and repeatabilities were validated; for HFLPME, extraction solvent, sample phase pH, agitation speed, extraction time and sample phase volume were investigated. Many active components were screened from three medicines. Some of them, such as scoparone, psoralen, bergapten, oxypeucedanin, imperatorin, ligustilide, were identified by MS. The target fishing factors of active components and the cell apoptosis rates of three cells under the medicines effect were researched. The binding sites of active groups on HepG-2 cells were preliminarily determined. The results demonstrated that HFCF-HPLC, coupled with HFLPME-HPLC, is a simple and universal approach to find bioactive components at the cellular level, determine their content and research traditional Chinese medicines (TCMs) entirety effect of multi-component and multi-target. The approach may provide us a new and good solution to clarify the material basis of anti-cancer effect and conduct personalized quality control for the components associated with efficacy in TCMs.