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Sellam, F., Harir, N., Khaled, M.B., Mrabent, N.M., Belkralladi, H., Tou, A., Diaf, M., Salah, R. and Moulessehoul, S. (2015) Immunohistochemical Examination of Cholecystokinin and Gastrin Receptors (CCK-2/Gastrin-R) Expression in Normal and Exocrine Cancerous Human Pancreatic Tissues. Pancreatology, 15, 661-666.
http://dx.doi.org/10.1016/j.pan.2015.09.019

has been cited by the following article:

  • TITLE: Role of p38 Signaling Pathway in Pentagastrin-Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT-29

    AUTHORS: Jiading Mao, Pei Wu, Jian Wu, Liang Tao, Ping Wu, Guang Yang, Wenwen Guo, Jun Wang

    KEYWORDS: Gastrin, p38, Proglumide, Proliferation Index, Apoptosis Rate, MAPK, Colorectal Carcinoma

    JOURNAL NAME: Journal of Cancer Therapy, Vol.7 No.6, June 14, 2016

    ABSTRACT: Objective: To investigate the effects and mechanisms of p38 signaling pathway in pentagastrin-regulated cell proliferation of colorectal carcinoma cell line HT-29. Methods: HT-29 cell line of colorectal carcinoma was in vitro incubated and divided into the control group, pentagastrin group, proglumide group, and pentagastrin + proglumide group. MTT reduction assay was performed to detect the proliferation status of HT-29 cell line and determine the optimal dosage of pentagastrin and proglumide. Annexin V-fluorescein isothiocyanate flow cytometry was used to detect the proliferation index (PI) and apoptosis rate (AR) of HT-29 cells. Reverse transcriptase polymerase chain reaction was performed to detect the mRNA expression of the pentagastrin receptor/cholecystokinin-B receptor (CCK-BR) and p38. The protein and phosphorylation levels of p38 were estimated by western blotting. Results: RT-PCR detection showed that CCK-BR mRNA was expressed in the HT-29 cell line. Pentagatrin improved HT-29 cell proliferation in dosage of 6.25 - 100 mg/L, and the optimal dosage of pentagastrin was 25.0 mg/L. Proglumide had no significant effect on the proliferation of HT-29 cells, but significantly inhibited the proliferation of HT-29 cells stimulated by pentagastrin when the dosage of proglumide was 8.0 - 128.0 mg/L, and the optimal dosage was 32.0 mg/L. The AR in the pentagastrin group was significantly lower than that in the control group and in the pentagastrin + proglumide group. The PI in the pentagastrin group was significantly higher than that in the control group and in the pentagastrin + proglumide group. P38 phosphorylation level in the pentagastrin group was significantly lower than that in the control group, and in the pentagastrin + proglumide group. There were no significant differences in the mRNA and protein expression of p38 in the control, pentagastrin, proglumide and pentagastrin + proglumide groups. Conclusion: Pentagastrin can improve proliferation of the CRC cell line HT-29 and inhibit apoptosis via the p38 signal transduction pathway. This mechanism may be associated with suppressed p38 protein phosphorylation level due to inhibition of proglumide, a gastrin receptor antagonist.