TITLE:
Metastasis of Mammary Carcinoma in Bitches: Evaluation of the Sentinel Lymph Node Technique
AUTHORS:
Hugo Enrique Orsini Beserra, Fabrizio Grandi, Rozany Mucha Dufloth, Luiz Gonzaga Porto Pinheiro, Hélio Amante Miot, Stephane Cássia Oliveira Rosa Vexenat, Noeme Sousa Rocha
KEYWORDS:
Metastasis, Sentinel Lymph Node, Mammary Neoplasia, Patent Blue, Female Dog
JOURNAL NAME:
Advances in Breast Cancer Research,
Vol.5 No.2,
April
8,
2016
ABSTRACT: In
recent years the development of extremely accurate techniques for the removal
of lymph nodes has been seen, such as the Sentinel Lymph Node Biopsy (SLNB) technique.
The technique is based on the staggered progression occurring in the lymphatic
drainage of neoplasias, and is currently the main prognostic and diagnostic
factor in women suffering from mammary carcinoma. This research aims at
assessing the sentinel lymph node biopsy accuracy in female dogs suffering from
mammary carcinoma. Forty-one dogs with cytological diagnosis of the tumor were
used in the research. After injecting a Patent Blue V dye, mastectomy was
conducted jointly with lymphadenectomy (for both sentinel and non-sentinel
lymph nodes). The material was then serially sectioned and microscopically
evaluated by routine H&E stain and immunohistochemical study with the
markers for pancytokeratin AE1/AE3 and myoepithelial cells CK14. The study
revealed that 46.3% (19/41) of the animals presented nodal metastasis. The
sensitivity and specificity values found for SLNB were respectively 89.5% and
100%, with a kappa coefficient of 0.90 and p