TITLE:
Osteogenesis of MC3T3 Preosteoblasts on 3D Bioactive Peptide Modified Nano-Macroporous Bioactive Glass Scaffolds
AUTHORS:
Colin Przybylowski, Mohamed Ammar, Courtney LeBlon, Sabrina S. Jedlicka
KEYWORDS:
Bioactive Glass, Bioactive Peptide, MC3T3, Osteogenesis
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.6 No.3,
July
3,
2015
ABSTRACT: Biointerface design that targets osteogenesis is a growing area of research with significant implications
in biomedicine. Materials known to either support or stimulate osteogenesis are composed
of a biomimetic ceramic material, such as bioactive glass. Bioactive glass is osteoproductive,
and the potential for osteoproductivity can be enhanced by the addition of proteins or other additives
designed to alter functionality. In addition, soluble growth factors are often added to osteogenic
culture on bioactive glasses, further intensifying the effects of the material. In this paper,
synthetic peptide combinations, covalently bound to a three-dimensional bioactive glass network,
are used to mimic the effects of the whole fibronectin and bone morphogenetic proteins (BMP) 2
and 9. Peptide-silanes possessing critical binding sequences from each of these proteins are synthesized
and used to decorate the surface of three-dimensional (3D) nano-macroporous bioactive
glass. MC3T3 preosteoblast cells are then assessed for differentiation on the materials in the absence
of soluble differentiation cues. MC3T3 preosteoblasts undergo enhanced differentiation on
the peptide-silane samples over the standard nano-macroporous bioactive glass, and the differentiation
capacity of the cells exposes only to peptide-silane surfaces approaches that of cells grown
in chemical differentiation induction media.