TITLE:
Pyrethroid Insecticide Resistance kdr Gene in the House Fly, Musca domestica (Diptera: Muscidae), in the United Arab Emirates
AUTHORS:
Mohammad Ali Al-Deeb
KEYWORDS:
House Fly, Pyrethroid, kdr, Resistance, UAE
JOURNAL NAME:
Agricultural Sciences,
Vol.5 No.14,
December
25,
2014
ABSTRACT: The house fly, Musca domestica L., is a very important insect
because of its role as a vector of several human, livestock, and poultry
diseases. Different groups of insecticides, including the pyrethroids, are
used to control house flies. The objective of the current study was to conduct
a survey of the kdr insecticide
resistance gene in the house fly population in the United Arab Emirates (UAE).
Flies were collected from five locations (Abu Dhabi, Dubai, Fujairah, Ajman,
and Ras Al- Khaymah). Genomic DNA was extracted and polymerase chain reaction
(PCR) amplification of specific allele (PASA) was performed. The maximum
percentage (70%) of homozygous genotype (kdr/kdr) occurred at
Ajman, followed by Dubai (59.5%), and the minimum percentage occurred at Ras
Al-Khaymah (14.3%). Resistant insects of the genotype kdr/kdr were detected in
all of the tested house fly populations. Ajman and Fujairah house fly
populations were in Hardy-Weinberg equilibrium. The resistance kdr allele was found at a high frequency (0.54 - 0.8) at all locations except at
Ras Al-Khaymah (0.21). Together, this study demonstrated that the pyrethroid
insecticide resistance kdr allele was found in UAE house fly
populations, strongly suggesting that a countrywide pyrethroid insecticide
resistance management program needs to be implemented.