TITLE:
In Vitro Germination and Plantlet Establishment of Wild Chamomile of Morocco Cladanthus mixtus (L.) Oberpr. and Vogt
AUTHORS:
Naima Harras, Ahmed Lamarti
KEYWORDS:
Asymbiotic Seed Germination, In Vitro Propagation, Basal Media, Cladanthus mixtus (L.)
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.18,
August
7,
2014
ABSTRACT:
In vitro seeds germination and
plantlets establishment of Cladanthus mixtus (L.) Oberpr. and Vogt were
studied in this report. A reliable protocol was developed for in vitro seed germination, multiplication and plantlet regeneration of Cladanthus
mixtus. The seeds were sterilized and cultured in different media. Among
the two basal media evaluated for symbiotic seed germination, gelled distilled
water was found to be the best with a high percentage of seeds germination
(100%) after 33 hours of culture. Seedlings were further transferred to
different types of media. Result observed after 4 weeks showed that MS medium
promotes the highest growth with an average of 2.75 ± 0.12 cm shoot length and
2.60 ± 0.29 shoots per explants, and the mean number of roots achieved 3.33 ± 0.17
root per explants with a length of 2.42 ± 0.16 cm. This study showed that macroelements
of MS (1962) medium is essential for in vitro shoot multiplication,
growth and rooting of shoots of Cladanthus mixtus L.